BRAF K601N/BaF3
CBP73386
                       
                         詢  價(jià)
                        
                        
                留 言
                        
                    產(chǎn)品描述
                    產(chǎn)品數(shù)據(jù)庫(kù)
                    
                | I. Introduction | |
| Cell Line Name: | BRAF K601N/BaF3 | 
| Host Cell: | Ba/F3 | 
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | 
| Application: | Anti-proliferation assay and PD assay | 
| Freeze Medium: | 90% FBS+10% DMSO | 
| Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin | 
| Mycoplasma Status: | Negative | 
| II. Background | |
| BRAF K601N lies within the protein kinase domain of the Braf protein (UniProt.org). K601N confers a gain of function on Braf, as indicated by increased phosphorylation of MEK and ERK in cell in culture and induction of cell proliferation and cell viability in culture. | |
| III. Representative Data | |
| 
				 1. WB of BRAF K601N/BaF3 
  | 
		|
| 
				 2. Sanger of BRAF K601N/BaF3 
 Figure 2. Sanger of BRAF K601N/BaF3  | 
		|
| 
				 3. Anti-proliferation assay  | 
		|
![]()  | 
		|
| 
				 Figure 3. CTG Proliferation Assay of BRAF K6D1N BaF3 Cells (C12).  | 
		|
            

