Gα15/HEK293
CBP71393
                       
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                | I. Background | |
| Gα15(GNA15)/HEK293:HEK293/Gα15 is a HEK293 cell line stably expressing the Gα15 alpha subunit protein which a Gq protein. It is used as a host cell for transfection expression of Gs and Gi/o -coupled receptors, the constitutively expressed Gα15 protein in the cells allows many transfected receptors which normally stimulate/inhibit the HEK293/Gα15. | |
| II. Introduction | |
| Host Cell: | 
				 HEK293  | 
		
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | 
| Freeze Medium: | 90% FBS+10% DMSO | 
| Culture Medium: | DMEM+10%FBS+ 200 μg/ml Hygromycin B | 
| Mycoplasma Status: | Negative | 
| Storage: | Liquid nitrogen immediately upon delivery | 
| Application(s): | 
				 Functional assay for Gα15(GNA15)receptor  | 
		
| Transducer: | N/A | 
| Ⅲ. Description of Host Cell Line | |
| Organism: | Human | 
| Tissue: | kidney | 
| Morphology: | Epithelial | 
| Growth Properties: | Adherent | 
| Ⅳ. Representative Data | |