BT474-Luc
CBP30103L
                       
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                | I. Introduction | |
| Host Cell: | BT-474 HER2 amplification | 
| Expressed gene: | Luciferase | 
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) | 
| Freeze Medium: | 90% FBS+10% DMSO | 
| Culture Medium: | DMEM+10%FBS+0.2%BT474 advance+2ug/ml puromycin | 
| Mycoplasma Status: | Negative | 
| Storage: | Liquid nitrogen | 
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human | 
| Tissue: | Mammarygland;breastduct | 
| Disease: | Ductal carcinoma | 
| Morphology: | Epithelial | 
| Growth Properties: | Adherent,patchy The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent | 
| III. Representative Data | |
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				 Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..  | 
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| IV. Stablity Test | |
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				 Figure. Stablity test results. BT474-Luc cells showed at least 32 passage stability with similar luminescence unit.  | 
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